RNA oligo
Synthetic RNA is widely used in research as a means for functional analysis and for the development of new therapeutic strategies; in recent years, the advent of synthetic siRNAs has made these molecules increasingly requested for their use in antisense technologies.
We have expanded our RNA oligo offer to always be alongside our customers and provide as much service as possible. as personalized as possible and close to the different experimental needs.
PORTFOLIO OLIGO RNA
Oligo with Desalted purifies
For oligos from 5 to 40 nucleotides there are 5 synthesis scales with "desalted" purification with guaranteed quantity delivered and independent of the length:
>/= 5 < 25 nmol
>/= 25 < 50 nmol
>/= 50 < 100 nmol
>100 nmol
Oligo with HPLC purification
For oligos from 2 to 80 nucleotides there are 6 synthesis scales with "HPLC" purification with guaranteed quantity delivered and independent of the length:
>/= 5 < 25 nmol
>/= 25 < 50 nmol
>/= 50 < 100 nmol
>/= 100 < 200 nmol
>/= 200 < 400 nmol
- An extensive selection of standard modifications (5 ', internal, 3')
- Quality control via Mass-check
- Shipping of lyophilized oligo, annealing buffer and protocol available on request.
- Delivery time 5-10 working days.
RNA LONGMERS
Bio-Fab also offers RNA oligos up to 140 nucleotides, HPLC purified! HPLC purification ensures that the delivered product is composed, for the most part, of full-length oligos. Mass-check control ensures that the final oligo is composed only of oligos at 100% of the desired length.
PLATE OLIGO
RNA oligos can also be delivered in 96-well plates.
For the oligos in the plate there are two synthesis scales with guaranteed quantity delivered and independent of the length:
≥ 10 < 25 nmol
≥ 25 < 50 nmol
RNA DUAL LABELED PROBES (RNA DLPs)
Our RNA Dual LabeledProbes have been optimized for use in real-time PCR. Since for this type of oligonucleotides the quality is of fundamental importance to obtain reproducible data, we worked to optimize the synthesis and the purification protocols in order to minimize the background noise. The most suitable quencher molecules were selected to cover the emission spectrum of the reporters; all in order to offer a high quality probe that works well in your identification experiments.
The wide choice of reporter-quencher combinations is suitable for both "probe hydrolysis" assays and those based on "probe hybridization" and can satisfy all requests for single or multiplex real-time PCR.
Furthermore, to eliminate any ambiguity about the quantities, for our RNA oligos, we have decided to use synthesis scales in nmoles. This is because the amount of moles is independent of the physical characteristics and sequence of each oligo and allows different oligos to be compared and diluted to the desired amount.
5 summary scales with guaranteed and length-independent delivered quantity:
≥ 1 < 5 nmol
≥ 5 < 10 nmol
≥ 10 < 20 nmol
≥ 20 < 30 nmol
≥ 30 < 50 nmol
NOTE:
- Advantage of oligos delivered in nmole: guaranteed and length-independent quantity per oligo from 18 to 40 mers. The OD260 value measures the total optical density of the nucleotides present in solution. For example:
1 OD of a 20nt oligo 5´CAT CGT ATT CGA TGC TAC GT 3´
is approximately 5 nmol.
1 OD di un oligo da 40nt: 5´CAT CGT ATT CGA TGC TAC GT CAT CGT ATT CGA TGC TAC GT 3´ is approximately2.5 nmol.
Consequently, with the same OD, the quantity of product delivered may vary significantly from one oligo to another. The use of nmoles per probe ranging from 18 to 40 nucleotides excludes any possible ambiguity on the quantities delivered. - The Reporter-Quencher 5 '- 3' and 3 '- 5' combinations are suitable for any commonly used Real Time PCR platform . However, do not hesitate to contact us for any combination or modification not present in the price list.
- HPLC purification included in the price and quality control by Mass-Check to always guarantee the highest quality of our products!
SMALL INTERFERING RNA (siRNA)
Small interfering RNA (siRNA) are RNA molecules between 20 and 30 nucleotides long that inhibit the expression of single genes through complementary nucleotide sequences. The siRNAs have a well-defined structure: a short molecule of double strand RNA (dsRNA) with a 5 'hydroxylated end and two protruding nucleotides at the 3' end.
Gli siRNApossono regolare l'espressione di specifici geni attraverso la loro complementarietà con gli mRNA causandone la distruzione e di conseguenza la non traslazione in proteina. Un numero crescente di screening basati sull’RNAi vengono progettati per identificare i geni coinvolti in importanti pathway metabolici: in particolare alcuni processi patologici dipendono proprio dall’attività simultanea di più geni: riuscendo a disattivarli tramite gli siRNA, si possono produrre molti benefici terapeutici.
Standard portfolio for siRNA / Duplex:
- The price is independent of the length and quantity delivered between 100 and 200 nmol for 11-40 nucleotides
- There is no extra cost for 3 'overhangs (max 2 nucleotides)
- There is an extra price for phosphorylation at both ends, regardless of scale
- All siRNA duplexes are HPLC purified and Mass Check tested
- They are shipped freeze-dried: see the instructions for resuspending them
- DNA / PTO and DNA / 2´OMe RNA chimeras are available
DOWNLOAD FORM SINTESI OLIGO RNA